Automation of CyQuant LDH Cytotoxicity Assay using Biomek i7

This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

Cell proliferation was assessed by measuring fluorescence following 120 hours of indicated compound treatment using the CyQUANT Cell

cyquant protocol Thinking that perhaps serum deprivation was not synchronizing the cells, I used Invitrogen's CyQUANT in order to determine cell proliferation

cyquant protocol following the same protocol and using the same lambda DNA standard for bTC3 cells and rat islets Values measured with the CyQUANT